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96-well filter plate with a pvdf membrane bottom  (Merck KGaA)

 
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    Structured Review

    Merck KGaA 96-well filter plate with a pvdf membrane bottom
    96 Well Filter Plate With A Pvdf Membrane Bottom, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/96-well+filter+plate+with+a+pvdf+membrane+bottom/96+well+filter+plate/pm37669293-161-19-22
    Average 90 stars, based on 1 article reviews
    96-well filter plate with a pvdf membrane bottom - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Evidence for preexisting prion substrain diversity in a biologically cloned prion strain.
    Article Snippet: Briefly, brain homogenate (1% w/v) was incubated in Gdn-HCl (Sigma-Aldrich, St. Louis, MO) ranging from 0 M to 3.5 M while shaking for one hour at room temperature. .. The concentration of Gdn-HCl was adjusted to 0.5 M prior to transferring to a 96-well filter plate with a PVDF membrane bottom (Merck Millipore, Co. Cork, Ireland). .. Samples were digested with PK (5 μg/mL; 1:100 PK:BH) for one hour at 37 ̊C (5 μg/ml; Roche Diagnostics, Mannheim, Germany) followed by incubation with phenylmethane sulfonyl fluoride (PMSF; MP Biomedicals, LLC, Salon, OH) for 20 minutes at room temperature.

    Article Title: Prion protein amino acid sequence influences formation of authentic synthetic PrP Sc .
    Article Snippet: Brain homogenate was diluted 1:10 (spinal cord homogenate diluted 1:5) into DPBS (Corning, Manassas, VA) from a 10% w/v brain or spinal cord homogenate and subsequently incubated in Gdn-HCl (1:3 diluted homogenate:Gdn-HCl dilution) with shaking for one hour at room temperature. .. The concentration of Gdn-HCl was adjusted to 0.5 M for all samples prior to plating onto a 96-well filter plate with a PVDF membrane bottom (Telling; Merck Millipore, Co. Cork, Ireland). .. Once samples were spun down and bound to the plate membrane, samples were digested with PK (5 μg/mL; 1:100 PK:BH) for one hour at 37 °C (5 μg/ml; Roche Diagnostics, Mannheim, Germany), followed by incubation with phenylmethane sulfonyl fluoride (PMSF; MP Biomedicals, LLC, Salon, OH) for 20 min at room temperature to halt PK digestion.

    Article Title: Efficient interspecies transmission of synthetic prions
    Article Snippet: .. Guanidine hydrochloride concentration was adjusted to 0.5 M for all samples prior to plating on a 96-well filter plate with a PVDF membrane bottom (Merck Millipore, Co. Cork, Ireland). .. Samples were dried at room temperature for one hour followed by digestion with PK (5 μg/mL; 1:100 PK:BH) at 37°C for one hour (5 μg/ml; Roche Diagnostics, Mannheim, Germany).

    Transferring:

    Article Title: Evidence for preexisting prion substrain diversity in a biologically cloned prion strain.
    Article Snippet: Briefly, brain homogenate (1% w/v) was incubated in Gdn-HCl (Sigma-Aldrich, St. Louis, MO) ranging from 0 M to 3.5 M while shaking for one hour at room temperature. .. The concentration of Gdn-HCl was adjusted to 0.5 M prior to transferring to a 96-well filter plate with a PVDF membrane bottom (Merck Millipore, Co. Cork, Ireland). .. Samples were digested with PK (5 μg/mL; 1:100 PK:BH) for one hour at 37 ̊C (5 μg/ml; Roche Diagnostics, Mannheim, Germany) followed by incubation with phenylmethane sulfonyl fluoride (PMSF; MP Biomedicals, LLC, Salon, OH) for 20 minutes at room temperature.

    Membrane:

    Article Title: Evidence for preexisting prion substrain diversity in a biologically cloned prion strain.
    Article Snippet: Briefly, brain homogenate (1% w/v) was incubated in Gdn-HCl (Sigma-Aldrich, St. Louis, MO) ranging from 0 M to 3.5 M while shaking for one hour at room temperature. .. The concentration of Gdn-HCl was adjusted to 0.5 M prior to transferring to a 96-well filter plate with a PVDF membrane bottom (Merck Millipore, Co. Cork, Ireland). .. Samples were digested with PK (5 μg/mL; 1:100 PK:BH) for one hour at 37 ̊C (5 μg/ml; Roche Diagnostics, Mannheim, Germany) followed by incubation with phenylmethane sulfonyl fluoride (PMSF; MP Biomedicals, LLC, Salon, OH) for 20 minutes at room temperature.

    Article Title: Prion protein amino acid sequence influences formation of authentic synthetic PrP Sc .
    Article Snippet: Brain homogenate was diluted 1:10 (spinal cord homogenate diluted 1:5) into DPBS (Corning, Manassas, VA) from a 10% w/v brain or spinal cord homogenate and subsequently incubated in Gdn-HCl (1:3 diluted homogenate:Gdn-HCl dilution) with shaking for one hour at room temperature. .. The concentration of Gdn-HCl was adjusted to 0.5 M for all samples prior to plating onto a 96-well filter plate with a PVDF membrane bottom (Telling; Merck Millipore, Co. Cork, Ireland). .. Once samples were spun down and bound to the plate membrane, samples were digested with PK (5 μg/mL; 1:100 PK:BH) for one hour at 37 °C (5 μg/ml; Roche Diagnostics, Mannheim, Germany), followed by incubation with phenylmethane sulfonyl fluoride (PMSF; MP Biomedicals, LLC, Salon, OH) for 20 min at room temperature to halt PK digestion.

    Article Title: Efficient interspecies transmission of synthetic prions
    Article Snippet: .. Guanidine hydrochloride concentration was adjusted to 0.5 M for all samples prior to plating on a 96-well filter plate with a PVDF membrane bottom (Merck Millipore, Co. Cork, Ireland). .. Samples were dried at room temperature for one hour followed by digestion with PK (5 μg/mL; 1:100 PK:BH) at 37°C for one hour (5 μg/ml; Roche Diagnostics, Mannheim, Germany).



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